英语原文共 7 页， 尿苷-胞苷激酶对尿苷和胞苷的磷酸化作用 摘要 以重组尿苷胞苷激酶和醋酸激酶为原料，合成了5-单磷酸尿苷(5-UMP)和5-单磷酸胞苷(5-CMP)。将大肠杆菌K12的ACK和UCK基因以及保加利亚乳杆菌ATCC 11842的ACK1、ACK2和ACK基因克隆到质粒pET-28a中，构建了重组质粒。以鸟苷5-三磷酸(GTP)为磷酸中间体，经磷酸乙酯再生得到重组大肠杆菌。优化了几个参数的作用，包括底物浓度，GTP浓度，温度和反应pH。如果尿苷或胞苷被来自大肠杆菌的ACK编码的胞苷激酶和来自保加利亚乳杆菌的ACK编码的磷酸化酶，则可以实现高效率。在37°C和pH 7.5条件下，用30 mM尿苷/胞苷和0.5 mM GTP 共1ml 时，5-UMP和5-CMP的最大转化率为97%。另外，5-UMP和5-CMP产物在反应体系中非常稳定，没有经历明显的降解。 关键词:5-单磷酸尿苷，5-单磷酸胞苷，尿苷-胞苷激�

英语原文共 25 页， 英语译文共 31 页， 资料编号：[257313]，资料为PDF文档或Word文档，PDF文档可免费转换为Word

英语原文共 12 页， 英语译文共 11 页， 资料编号：[257296]，资料为PDF文档或Word文档，PDF文档可免费转换为Word

英语原文共 6 页， 英语译文共 7 页， 资料编号：[257322]，资料为PDF文档或Word文档，PDF文档可免费转换为Word

英语原文共 5 页， 英语译文共 10 页， 资料编号：[257319]，资料为PDF文档或Word文档，PDF文档可免费转换为Word

英语原文共 15 页， 非靶向血浆代谢组学分析精液异常男性不育症 摘要 精液异常（SA）男性不育症已经成为世界性生殖健康问题。侵入性检查（例如睾丸活检）和人工密集型的精液采集法严重影响影响了男性不育的诊断。此外，男性不育的发病机理和生物学解释尚不清楚。本文采用GC-MC对84例精液异常（SA）患者，包括畸精症（TE）21例，弱精子症（AS）23例，少精子症（OL）20例，无精子症（AZ）20例和年龄相同的健康对照组（HC）29例进行判别分析，寻找潜在的生物标志物。采用多元统计法（偏最小二乘判别分析，PLS-DA）和单变量统计法（方差分析，ANOVA）比较TE与HC、AS与HC、OL与HC、AZ与HC的差异，得到了23个生物标志物。基于这些生物标志物，最相关的途径主要与碳水化合物、氨基酸和脂质的代谢有关。SA男性不育中的主要代谢改变�

英语原文共 11 页， 转录组分析证实金丝桃素的抗肿瘤机制 HOS胶质母细胞瘤细胞系的研究 关键词：金丝桃素 HOS骨肉瘤系转录组分析 细胞凋亡 目的：贯叶连翘的金丝桃素对多种恶性细胞有细胞毒性作用，但介导这种作用的基因表达模式尚不清楚。在这里，我们试图分析HOS胶质母细胞瘤(GBM)细胞系对HYP的反应。 材料与方法：用HYP处理HOS骨肉瘤株。用MTT法和AnnexinV/PI法测定细胞毒性。通过高通量测序获得基因表达谱。对差异表达基因(DEGS)进行富集分析)。预测上游转录因子和调控DEGS的微RNA。计算DEGS对GBM患者生存的影响。进行蛋白质-蛋白质相互作用分析，以获得关键的改变基因。评价HYP治疗对免疫应答的可能影响。 关键发现：HOS骨肉瘤株HYP的IC50测定为1.5mu;g/ml。细胞死亡的主要类型是凋亡。共发现312个DEGS。对受影响的基因�

英语原文共 23 页， 10.3 Management of 3D Spatial Data In order to manage a 3D geo-information, at least the use of databases and their management systems (DBMS) are required. Object-relational modeling is the most common since relational databases are not very appropriate for storing spatial data. The object-oriented database approach faces the problem that the general acceptance and knowledge is not available so far(Connolly and Begg, 2002). The field of geo information adopts both approaches and comprises them into Object-Relational DBMS (Shekar andChawla, 2003). As stated in section 10.2, the additional integration of spatial extensions iS compilsory for GIS applications. Furthermore, because operations of 3D functionality are different from 2D, a reasonable concept of data storage is inevitable. Therefore, the two aspects of 3D geometry and 3D topology have to be taken into account. Geometry holds 3D coordinates of objects and topology holds their spat

英语原文共 8 页， Chapter 59 Research on GIS 3D Visualization Based on ArcGIS and SketchUp Abstract Many specialized agencies start to do basic research of 3D GIS, and a lot of prototypes of 3D GIS were developed today. But, due to most of the traditional modeling software have many defects. So the method of ArcGIS combined with SketchUp is brought forward as a new idea in GIS 3D visualization because ArcGIS has powerful spatial analysis functions, while SketchUp is easy to learn and models quickly. This paper summarized the research status both home and abroad, discussed the application of the technology in various fields, analyzed its advantages and disadvantages, and then explored the development prospect of it. Keywords ArcGIS · SketchUp · GIS · 3D visualization 59.1 Introduction With 3D GIS becoming increasingly prevalent, the demand from users is on the increase too: 3D GIS should not only achieve the 3D displaying, but also t

英文原文： Addressing environmental design in interior architecture education: Reflections on the interior design studio Abstract Today , the fluid and multi - layered structure of the environment demands design professions acquirea broader perspective in conjunction with a more expanded , cross - boundary knowledge and skills . This paper mainly concentrates on the structure of interior architectural studies and the significance of environmental design . The study presents a descriptive reading through the experiences in the interior design studio , and in this sense evaluates the outcomes within the educational process . The aim is to discuss the relationship between the interior / exterior or environment / building through the ideas created in the interior design studio . The key points are elucidated through examples of student work from two different interior architecture departments in Istanbul . The reflections upon developing the knowledge of “environ