2021-12-20 21:14:14

英语原文共 6 页

1. 介绍

Slaughterhouse waste water can cause serious pollution of the surrounding water environment if it is discharged directly and without disposal. The health of humans, livestock and poultry, and other aquatic organisms can be seriously endangered because of the large number of virulence factors and microorganisms in the waste water that are harmful to human health. If slaughter wastewater is drained into the surrounding water, the dissolved oxygen in the water will be rapidly depleted, causing the fish and aquatic organisms to die of lack of oxygen, because the outward slaughtering wastewater containing harmful substances are mostly easy biodegradable organic matter. At the same time, pathogenic microorganisms will also be produced in large quantities with the waste water discharged into the water, which seriously endangers the health of human beings. Therefore, it is necessary to deal with the wastewater from slaughtering meat processing, remove the pollution caused by waste water and completely eliminate the harm of water environment, so as to protect the ecological environment and human health.


Among the various methods proposed for the removal of these compounds, those based on microbial activities are of greater priority. Microbial treatment method is a low cost, no environmental pollution and thorough purification method compared with chemical treatment and physical treatment method in the treatment of slaughterhouse wastewater, will be a main method to solve the slaughterhouse wastewater pollution in the future. The most important is that to have excellent ability of degrading bacteria in microbial treatment method in the process of implementation, using microorganism to degrade organic pollutants in slaughter wastewater is the better method.


Up to now, a large number of bacterial strains capable of degrading COD have been isolated. Most reports have shown that COD-degrading bacteria isolated from different contaminated sites are belonging to various bacterial species, such as ammonia bacteria, nitrifying bacteria and denitrifying bacteria. Overall, slaughter wastewater contains a large amount of nitrogen-containing organic matter such as blood, animal internal organs and hair, and is easy to breed a large number of harmful organisms. Rapid and efficient degradation of slaughter wastewater is of great significance for environmental protection.


In this study, six high COD degrading bacteria were isolated from slaughter wastewater and identified preliminarily and investigated their ability to remove COD from water. Then, one of them with highest ability to remove COD from water was chosen, which of their physiological and biochemical characteristics were preliminarily studied in this paper.


2. 材料和方法


The slaughter wastewater and sludge contaminated with COD strain were the source for bacterial isolation. Samples were collected into 1000 ml sterilized glass bottles from eight sites in a Hunan meat product Co., Ltd on June 2016, and immediately carried to the laboratory and stored in a refrigerator prior at 4 C to use.

受COD污染的屠宰废水和污泥是细菌分离的来源。样品于2016年6月从湖南某肉制品有限公司8个现场抽取1000 ml保存在无菌玻璃瓶,立即送往实验室,在4℃下放入冰箱保存使用。


The slaughterhouse wastewater and sludge mixed samples were taken for 10 ml and filtered with gauze respectively. The samples were diluted and diluted to 10-1 , 10-2 , 10-3 , 10-4 , 10-5 , 10-6 , 10-7 respectively. In the sterile operating table, samples with different dilution concentrations were evenly coated in LB solid medium for 200 lL, and cultured in 32 C incubator for 5–7 d.

将屠宰场废水和污泥混合样品分别取10ml,用纱布过滤。将样品分别稀释至10-1、10-2、10-3、10-4、10-5、10-6、10-7。在无菌手术台中,将不同稀释浓度的样品均匀涂布于200ml的LB固体培养基中,在32 C培养箱中培养5-7 天。


The colonies were randomly picked enrichment lines separation, then were isolated single colonies obtained further separated to obtain single colonies of pure purification line, then pick a single colony is connected to the LB liquid medium, then add 4 C refrigerator spare.



Ammonium sulfate was added to LB culture medium, and the concentration of ammonium sulfate in culture medium was 0.3 g/100 ml under aseptic condition and was used to replace slaughterhouse wastewater, because it was closer to the content of reducing substance in real slaughter wastewater. The 10 ml medium with purified strains were isolated from the slaughter waste water were inoculated in 90 ml LB medium, shaker culture at 32 C, at regular intervals sampling and determination of COD in the medium by potassium permanganate method to calculate the degradation rate and draw the degradation curve.

在LB培养基中添加硫酸铵,在无菌条件下,培养基中硫酸铵浓度为0.3 g/ 100ml,由于硫酸铵更接近实际屠宰废水中还原性物质的含量,所以被用于代替屠宰场废水。10毫升介质与屠宰废水的纯化菌株接种在90毫升LB培养基,在32 ℃摇床中培养,定期采样和并用高锰酸钾法测定COD降解率计算和画出变化曲线。


The bacteria with the highest degradation rate of COD were observed by colony morpHology and Gram staining. Preliminary identification of the strains was conducted according to Bergeyrsquo;s Manual of Systema Bacteriology. Genetic identification of the isolated bacteria was done using 16S rDNA. Bacterial DNA was extracted using Ezup Column Bacteria Genomic DNA Purification Kit, and bacteri


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